Strategies for production of active eukaryotic proteins in bacterial expression system
نویسندگان
چکیده
منابع مشابه
Dictyostelium discoideum--a promising expression system for the production of eukaryotic proteins.
In general, four different expression systems, namely, bacterial, yeast, baculovirus, and mammalian, are widely used for the overproduction of biochemical enzymes and therapeutic proteins. Clearly, bacterial expression systems offer ease of maneuverability with respect to large-scale production of recombinant proteins, while, a baculovirus expression system ensures proper protein modifications,...
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Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...
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ژورنال
عنوان ژورنال: Asian Pacific Journal of Tropical Biomedicine
سال: 2012
ISSN: 2221-1691
DOI: 10.1016/s2221-1691(11)60213-x